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cDMD 4-5a [30-1, cDMD4-5a]
cDMD 4-5a [30-1, cDMD4-5a]
規(guī)格:
貨期:
編號:B243146
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 cDMD 4-5a [30-1, cDMD4-5a]
商品貨號 B243146
Designations cDMD 4-5a [30-1, cDMD4-5a]
GenBank Number

M18533

Species Homo sapiens, human
Depositors LM Kunkel
Applications
For plasmid amplification, use a lac Iq host.
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.850000023841858
Tissue: fetal muscle
Gene product: dystrophin (muscular dystrophy, Duchenne and Becker types), includes DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272 [DMD]
Alleles: U2, A1, A2, C1, T1, U1, A1, A1, A2, A2, B1, B2, C2, D2, R1, R2, T1, T2, T2, T3, D1
Insert Size (kb) 1.850
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
For plasmid amplification, use a lac Iq host.
Inserted into EcoRI site with 5' end closer to T7 promoter. Contains internal HincII and BglII sites.
ATCC 57670 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not needed for ATCC 57670, but is for ATCC 57671.
Of the DMD cDNA clones, 1-2a includes nucleotides 1-1538; 2b-3: 1455 to approximately 2600; 4-5a: 2600-4550; 5b-7: 4400-6900; 8: 6900-7800; 9-14: 7800-13900 (in base pairs from the 5' end of the cDNA).
Includes probes 4 and 5a. Detects genomic HindIII fragments of 7.3, 11, 20, 5.2, 12, 4.7, and 18 kb.
References

Lindlof M, et al. Gene deletions in X-linked muscular dystrophy. Am. J. Hum. Genet. 44: 496-503, 1989. PubMed: 2929594

Den Dunnen JT, et al. Topography of the Duchenne muscular dystrophy (DMD) gene: FIGE and cDNA analysis of 194 cases reveals 115 deletions and 13 duplications. Am. J. Hum. Genet. 45: 835-847, 1989. PubMed: 2573997

Lucotte G, et al. Molecular deletion patterns in Duchenne muscular dystrophy patients. Ann. Genet. 32: 214-219, 1989. PubMed: 2610487

Dominguez-Steglich M, et al. The dystrophin gene is autosomally located on a microchromosome in chicken. Genomics 8: 536-540, 1990. PubMed: 2286374

Koenig M, et al. Complete cloning of the Duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals. Cell 50: 509-517, 1987. PubMed: 3607877

Koenig M, et al. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein. Cell 53: 219-228, 1988. PubMed: 3282674

Louis M Kunkel, personal communication

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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