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lambdaF6
lambdaF6
規(guī)格:
貨期:
編號(hào):B242271
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) lambdaF6
商品貨號(hào) B242271
Designations lambdaF6
GenBank Number

M12949

Species Homo sapiens, human
Vector
Construct size (kb): 42.79999923706055
Insert
DNA: genomic
Insert lengths(kb): 13.30000019073486
Tissue: F8 cell line (T-prolymphocytic leukemia)
Gene product: T cell receptor, gamma, variable region V3 [TCRGJ1]
Insert Size (kb) 13.300
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): KpnI--17.0, 11.0, 4.8, 3.6, 1.8 (doublet), 1.5; PvuI--22.0, 13.0, 7.8; SacI--20.0, 9.1, 4.4 (doublet), 4.1; SmaI--20.0, 11.0, 6.2, 5.8; XhoI--20.0, 14.0, 9.1.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--3.3, 5.1, 10.5.
Contains a productive rearrangement of Vgamma3 and Jgamma1. The insert begins about 0.5 kb upstream of pseudogene Vgamma1 and extends 3.6 kb beyond J1.
Additional GenBank/EMBL accession numbers: M12953, M13429.
References

LeFranc MP, et al. Diversity and rearrangement of the human T cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45: 237-246, 1986. PubMed: 2938743

Marie-Paule Lefranc, personal communication

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