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FN771
FN771
規(guī)格:
貨期:
編號(hào):B242202
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 FN771
商品貨號(hào) B242202
Designations FN771
GenBank Number

M10905

Species Homo sapiens, human
Depositors M Chu
Applications
The coding sequence includes part of the fibrin binding domain, the 3' region showing species-specific variation, and a conserved cysteine.
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.299999952316284
Tissue: fibroblast ATCC CRL 1106 cell line
Gene product: fibronectin 1 [FN1]
Alleles: A2, E2, A1, B2, D2, B1, D1, E1
Insert Size (kb) 1.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains the following restriction sites (number): EcoRI (1), HindIII (1), HaeII (1).
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.0, 0.3; HindIII--5.7; PstI--5.7; EcoRI/HindIII--4.3, 0.9, 0.3, 0.2; BamHI--5.7.
The insert encodes the 3' 500 bp of the coding sequence and 3' untranslated sequences. The coding sequence includes part of the fibrin binding domain, the 3' region showing species-specific variation, and a conserved cysteine.
This probe detects a mRNA of 7.8 kb in human fibroblasts.
References

Bernard MP, et al. Human cellular fibronectin: comparison of the carboxyl-terminal portion with rat identifies primary structural domains separated by hypervariable regions. Biochemistry 24: 2698-2704, 1985. PubMed: 2992573

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沈經(jīng)理 19548299266 2662369050
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