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cos203
cos203
規(guī)格:
貨期:
編號:B242187
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 cos203
商品貨號 B242187
Designations cos203
Depositors D Kioussis
Biosafety Level 1
Vector Information
Size (kb): 10.1999998092651400
Vector: cos203 (cosmid)
Promoters: Promoter HSV TK
Marker(s):ampR,hygR
Construct size (kb): 10.19999980926514
Features: marker(s): ampR, hygR
promoter: HSV TK
replicon: pMB1
terminator: none
enhancer: none
Applications
shuttle vector
vector for constructing genomic libraries
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--10.05; XhoI--10.05; BglII--uncut.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The hygromycin resistance gene is expressed under the control of the HSV-TK promoter with a polyadenylation signal. This is not an expression vector.
The cloning sites were derived from the M13mp10/pUC12 polylinker with the EcoRI site having been destroyed.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C
References

Kioussis D, et al. Expression and rescuing of a cloned human tumour necrosis factor gene using an EBV-based shuttle cosmid vector. EMBO J. 6: 355-361, 1987. PubMed: 3034585

Dimitris Kioussis, personal communication

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