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Filamoeba nolandi Page
Filamoeba nolandi Page
規(guī)格:
貨期:
編號:B241979
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Filamoeba nolandi Page
商品貨號 B241979
Strain Designations NC-AS-23-1
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
sediment core 23 nautical miles off coast of Wilmington, NC, 1993
Product Format freeze-dried
Type Strain no
Medium ATCC® Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Temperature: 25.0°C
Duration: grown with Enterobacter aerogenes ATCC 13048 and mixed bacteria
Protocol: ATCCNO: 50430 SPEC: Add 1 ml bacterized ATCC medium 802 (inoculated 24 hours previously with ATCC 27889) to the inner shell vial. Transfer the filter paper plug aseptically from the vial with a sterile forceps to 10 ml bacterized ATCC medium 802 in a 25-ml flask. Incubate at 25C with the cap screwed on tightly.
Subcultivation
Protocol: ATCCNO: 50430 SPEC: Add 1 ml bacterized ATCC medium 802 (inoculated 24 hours previously with ATCC 27889) to the inner shell vial. Transfer the filter paper plug aseptically from the vial with a sterile forceps to 10 ml bacterized ATCC medium 802 in a 25-ml flask. Incubate at 25C with the cap screwed on tightly.
Cryopreservation
Cryoprotective Solution

DMSO ?????????????????????????????????????????????????????????????????????????????????? 2.0 ml

Fresh growth medium w/o bacteria???????????????????????????????? 8.0 ml

1.???? Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat.

2. ?? Harvest cells from a culture that is at or near peak density by filtration and centrifugation at 800 x g for 5 min.

3.???? Adjust the concentration of cells at least 2 x 106/ml in fresh medium.

4.? ?? Mix the cell preparation and the cryoprotective solution in equal portions.

5.? ?? Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

6. ??? Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately -1°C/min.) ?

7.? ?? Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator.

8.? ?? To establish a culture from the frozen state place the vial in a 35°C water bath.? Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.?? Immediately after thawing, do not leave in water bath, aseptically remove the contents of the ampule and inoculate into a T-25 tissue culture flask containing 10 ml ATCC medium 802 bacterized with Klebsiella pneumoniae subsp. pneumoniae (ATCC? ?700831).

9.     Incubate at 25°C with the cap screwed on tightly.

10.   Once the culture is established, vigorously agitate the flask and aseptically transfer 0.5 ml to 10.0 ml of bacterized ATCC medium 802.

11.   Follow the protocol for maintenance of culture.

Name of Depositor TK Sawyer
Year of Origin 1993
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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