久久精品女人天堂?V麻,久久99精品久久久久久齐齐,国产精品天干天干在线观看,?级毛片久久免费观看,国产美女在线精品免费观看,日韩欧美啪啪一区二区毛片,久久精品99国产亚洲?v成人,最新熟妇精品国产偷自在线,国产老妇多毛精品一区二区

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > Trypanosoma cruzi Chagas
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
Trypanosoma cruzi Chagas
Trypanosoma cruzi Chagas
規(guī)格:
貨期:
編號(hào):B241907
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Trypanosoma cruzi Chagas
商品貨號(hào) B241907
Strain Designations ERNESTINA CL44
Application
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Type Strain no
Medium ATCC® Medium 1029: LIT medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
Cryopreservation
1.?? Harvest cells from several cultures in very late logarithmic to early stationary phase of growth.? Vigorously agitate to suspend the cells.

2.?? Aseptically transfer the cell suspension to 15 ml plastic centrifuge tubes.

3.?? Centrifuge at ~800 x g for 5 min.

4.?? While cells are centrifuging, prepare a 10% solution of DMSO in complete ATCC Medium 1029.? Cool on ice.

5.?? Remove the supernatant and pool the cell pellets to the final volume desired with fresh growth medium.

6.?? Combine the cell suspension with an equal volume of 10% DMSO cryoprotectant solution (prepared in step 4) to yield a final concentration of 5% DMSO.

7.?? Dispense in 0.5 ml aliquots to 1.0-2.0 ml Nunc vials (special plastic vials for cryopreservation).

8.?? Place the vials in a controlled rate freezing unit.? From room temperature cool at -1°C/min to -40°C.? At -40°C, plunge ampules into liquid nitrogen.? Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.). ?

9.?? Store ampules in a liquid nitrogen refrigerator until needed.

10.????????? To establish a culture from the frozen state, place a frozen ampule in a 35°C water bath just enough to cover the frozen material.? Allow the ampule to thaw completely (2-3 min).

11.????????? Immediately after thawing, aseptically remove the contents and transfer to a T-25 tissue culture flask containing 10 ml of fresh complete ATCC medium 1029.

12.Screw the cap on tightly and incubate at 20-25°C.??? Observe the culture daily and transfer when numerous trophozoites are observed.?????????

Name of Depositor JA Dvorak
Special Collection NCRR Contract
References

Nozaki T, Dvorak JA. Intraspecific diversity in the response of Trypanosoma cruzi to environmental stress. J. Parasitol. 79: 451-454, 1993. PubMed: 8501607

Nozaki T, Dvorak JA. Trypanosoma cruzi: flow cytometric analysis of developmental stage differences in DNA. J. Protozool. 38: 234-243, 1991. PubMed: 1880761

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
抚顺市| 海宁市| 桂阳县| 秭归县| 车致| 山阴县| 六安市| 新郑市| 江安县| 呼伦贝尔市| 吴桥县| 富蕴县| 皋兰县| 甘孜| 珲春市| 察雅县| 通州市| 温泉县| 遂溪县| 阿图什市| 木兰县| 平乡县| 措勤县| 新竹县| 吴忠市| 孟州市| 武隆县| 定西市| 当涂县| 岳西县| 肃北| 农安县| 固原市| 荔波县| 改则县| 邵阳县| 邢台县| 阳东县| 霍州市| 那坡县| 伊春市|