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pHM6
pHM6
規(guī)格:
貨期:
編號(hào):B241464
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pHM6
商品貨號(hào) B241464
Designations pHM6
GenBank Number

Z29675

Species Yersinia enterocolitica subsp. enterocolitica (Schleifstein and Coleman) Frederiksen
Depositors A Rakin
Applications
in another host, produces protein ferric yersiniabactin uptake receptor
Vector
Construct size (kb): 5.0
Insert
DNA: genomic
Insert lengths(kb): 2.650000095367432
Gene product: ferric yersiniabactin uptake receptor [fyuA]
Target Gene: ferric yersiniabactin uptake receptor
Insert Size (kb) 2.650
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): PstI/SalI--2.7, 2.4; EcoRI--3.2, 1.9; EcoRV--4.5, 0.5.
Insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--1.82; EcoRV--1.34, 1.79; PvuI--1.33; SphI--1.40.
The insert can be expressed in E. coli in the presence of T7 RNA polymerase and iron-supplemented media.
Expression from the Yersinia promoter is induced by growth in iron-limiting conditions in Y. erterocolitica as well as in E. coli.
Production of the receptor results in pesticin sensitivity (pstS).
References

Rakin A, et al. The pesticin receptor of Yersinia enterocolitica: a novel virulence factor with dual function. Mol. Microbiol. 13: 253-263, 1994. PubMed: 7984105

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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