| 產(chǎn)品名稱(chēng) |
A717 |
| 商品貨號(hào) |
B163891 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for glycated human serum albumin. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against glycated serum albumin (glycoalbumin) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against glycated serum albumin (glycoalbumin) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with native glycated human serum albumin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for glycated human serum albumin. It does not react with unglycated albumin. |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG , IgG |
| Name of Depositor |
Exocell, Inc. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Cohen MP. Treatment of complications of diabetes with substances reactive with the fructosyl-lysine structure in glycated albumin. US Patent 5,518,720 dated May 21 1996
Cohen MP, Hud E. Production and characterization of monoclonal antibodies against human glycoalbumin. J. Immunol. Methods 117: 121-129, 1989. PubMed: 2913157
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