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17/14
17/14
規(guī)格:
貨期:
編號(hào):B163599
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 17/14
商品貨號(hào) B163599
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against theophylline
Cellular Products
immunoglobulin; monoclonal antibody; against theophylline
Tumorigenic Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with theophylline-8-KLH.
Spleen cells were fused with NS-1 myeloma cells.
Complete Growth Medium
Culture medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;
fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor E.I. du Pont de Nemours & Co.
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Geltosky JE. Hybridoma cell lines and monoclonal antibodies to theophylline. US Patent 4,524,025 dated Jun 18 1985

Geltosky JE. Hybridoma cell lines and monoclonal antibodies to theophylline. US Patent 4,521,510 dated Jun 4 1985

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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